Transcriptomic profiling of C57BL/6 tumor microenvironment following immunotherapy by bulk RNA-seq

This project aims to decipher the immunomodulatory effects of immunotherapy on the tumor microenvironment (TME) in C57BL/6 mice bearing B16-F10. Tumors were harvested after three doses of treatment for bulk RNA-seq analysis. Data will reveal key transcriptional changes in immune and stromal cells. Processed reads were aligned to GRCm39 genome, and differential expression analysis was performed with DESeq2. Findings may provide insights into resistance mechanisms and combinatorial therapy design for solid tumors.