Generation and characterization of a variant of Thermostable Moloney Murine Leukemia Virus Reverse Transcriptase Enzyme

Reverse Transcriptase (RT) enzymes have been extensively utilized, especially in Polymerase Chain Reaction (PCR). Many viruses that cause infections worldwide contain ribonucleic acid (RNA), and for their molecular detection, it is essential to convert RNA to DNA using RT. This study aimed to create and characterize a thermostable Moloney Murine Reverse Transcriptase (MMLV-RT) enzyme by using consensus sequences from the latest MMLV-RT sequence database.