Single-cell genome-wide analysis of macula densa cell gene expression in control and physiological stimulatory conditions

Single cell based gene expression analysis of the rare kidney cell type, the macula densa in control and physiological stimulatory conditions. The hypothesis tested in the study was that macula densa cells utilize unique and cell-specific molecular networks that play important roles in sensory, regulatory, and secretory functions, and in the physiological stimulation of these cells by environmental clues such as low salt condition. Results provide important information of the identity, biological and physiological functions of macula densa cells consistent with their chief role in the regulation of kidney functions. RNA obtained from freshly isolated single mouse macula densa cells. Mice were subjected to 14 days of normal salt (control) or low salt (LS) diet.