Figure S1 - Biochemical Characterization of Hypothetical Proteins from Helicobacter pylori

SDS-PAGE analysis of Coomassie-stained proteins isolated using derivatized gold nano-particles. In four of the five instances only a single intense band is observed. In lane 5, two bands were observed, which were identified using mass spectrometry as HP0803 and HP0959. GTP cyclohydrolase activity was observed for HP0959, however HP0803 resisted multiple attempts at cloning and expression in E. coli, it was not pursued further. HP0803 only has one know protein domain, DUF3943 (PF13084), and so its reason for binding to the derivatized nano particle coated with guanosine-5'-triphosphate (GTP) and dihydroneopterin triphosphate is unexplained. (Data for HP0310 not shown.). (DOC)