Welfare-related assessments.

a Applied for non-invasive procedures (electrocardiogram, bioluminescence imaging) or for terminal bleeding. b Includes 3 days to acclimation before study star/ and statement to follow 3R's principle. c Includes anticipated end points in parasitaemia peak or with severe adverse effects, refined oral administration or oral volume up to 50 μL/animal or 7 to 10 days to acclimation before study starts. d Methods: tribromoethanol followed by decapitation; dyethyl-ether followed by cervical dislocation; thiopental overdose; CO2 inhalation. e CO2 inhalation in all cases. Welfare-related assessments.