Effect of Nur77 on cholesterol content in THP-1 macrophage-derived foam cells.

THP-1 macrophage-derived foam cells were divided into six groups and treated with DMSO (Control) or 10 µg/ml Csn-B (Csn-B) for 24 h, were treated with control (pcDNA-Mock) or recombinant plasmid over-expressing Nur77 (pcDNA-Nur77) for 48 h, and were treated with negative control (NC) or siRNAs against Nur77 (si-Nur77) for 48 h, respectively. Cellular cholesterol and cholesterol ester were extracted as described above. HPLC was performed to determine the cellular total cholesterol (TC), free cholesterol (FC) and cholesterol ester (CE). The results are expressed as mean ± S.D. from three independent experiments, each performed in triplicate. *p<0.05 vs. control group, #p<0.05 vs. pcDNA-Mock group, and p<0.05 vs. NC group.