The mutation efficiency of MSTN-ZFN (set 1) in different mammalian species.
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*The mutant efficiency is calculated by the mutant TA-cloning of PCR products of mixed cells/total sequencing number. The mismatch affected the ZFNs cut-efficiency dramatically. Lowercase letters were represented different nucleotides according to the targeting sequence of bovine. The bold italics represent the spacer nucleotides between the two ZFN monomers.
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2015-12-02



