Cajanus cajan strain:Asha (ICPL87119) Genome sequencing and assembly. Cajanus cajan strain:Asha (ICPL87119)

DNA denaturation-renaturation (Cot) based sequence discovery represents a powerful means to selectively and efficiently fractionate low-copy and repetitive genomic sequences for molecular characterization. Renaturation kinetics was used to classify the pigeonpea genomic DNA into highly repetitive (Cot 1), moderately repetitive (Cot 10) and single/ low-copy (Cot 100) sequences that were subsequently cloned to produce Cot 1, Cot 10 and Cot 100 libraries. Homology searches against Genbank nr and EST databases confirmed that Cot 1 library was enriched in high-copy number (retrotransposons, rDNA and other dispersed repeats) and the Cot 100 library was enriched in low-copy sequences. Cot 10 library was found to represent both low copy and high copy DNA sequences.