Dlx1/2-Dependent Expression of Meis2 Promotes Neuronal Fate Determination in the Mammalian Striatum [RNA-seq]

Purpose:To asses changes in gene expression profiles from the E14.5 littermate controls LGE and Meis2-CKO mice. Methods:The LGEs from E14.5 Meis2-CKO mutant and Control brains (n = 4 per group) were dissected, and RNA was isolated with the Direct-zol RNA Miniprep kit (Zymo, catalog #R2050) following the manufacturer's instructions. Results: Identified 161 genes with downregulated RNA expression and 94 genes with upregulated RNA expression LGE mRNA profiles of E14.5 old control (Ctrl) and Meis2-CKO mice研究目的：本数据集旨在评估E14.5天同窝对照侧脑室隆起区（Lateral Ganglionic Eminence, LGE）与Meis2-CKO小鼠的基因表达谱变化。 实验方法：分别剖取E14.5天Meis2-CKO突变型与对照组小鼠脑组织中的侧脑室隆起区（Lateral Ganglionic Eminence, LGE）组织（每组n=4），依照制造商说明书使用Direct-zol RNA Miniprep试剂盒（Zymo，货号#R2050）提取总RNA。 实验结果：在E14.5天老年对照组（Ctrl）与Meis2-CKO小鼠的LGE组织mRNA表达谱中，共鉴定出161个RNA表达下调基因与94个RNA表达上调基因。