Data for: The response and recovery of non-targeted fish species after rotenone eradication of an invasive species

This dataset contains environmental DNA (eDNA) data and associated sampling information used to assess the ecological consequences of a rotenone-based eradication of invasive Northern pike (Esox lucius) and the recovery of native fish species. The study was conducted in southern Norway and includes data collected over a two-year period spanning pre-treatment, treatment, and post-treatment phases (2021-09-30 – 2023-10-11). Water samples were collected across multiple habitats, including lake, tributary, and marine environments. Species presence was assessed using PCR-based eDNA detection. The dataset includes:(1) PCR detection data (binary presence/absence) for four fish species: - Northern pike (Esox lucius) - European eel (Anguilla anguilla) - Brown trout (Salmo trutta) - Three-spined stickleback (Gasterosteus aculeatus) This data is used for the main analysis (2) Geographic coordinates and habitat classification for all sampling stations. This data is used to create maps used in the publication. Each PCR observation represents one replicate (triplicate A–C) for a given sample, species, station, and sampling date. Time is expressed relative to the start of rotenone treatment (2021-11-02), allowing direct analysis of temporal dynamics. The dataset supports analyses of:- Species-specific recovery dynamics following disturbance- Effects of habitat and connectivity on re-colonization- Differences in resilience linked to species life-history traits The data are associated with the manuscript:"Ecological consequences of rotenone treatment and recovery dynamics of native fish species assessed using eDNA", submitted to Ecological Applications. All variables, units, and dataset structure are described in the accompanying metadata file. Code used for all analyses and figures is available in a separate GitHub repository and archived on Zenodo (https://github.com/lars-m-k/eDNA-pike-rotenone). Contact:Lars Mørch Korslund - [e-mail]