m6A modification in Herceptin resistant cells [MeRIP-Seq]

We report the application of specific antibodies and high-throughput sequencing technologies (methylated RNA immunoprecipitation sequencing, MeRIP-seq) for high-throughput profiling of m6A modifications in breast cancer herceptin resistant cells. We generated maps of m6A modified transcripts in BT474 and BT474.TtzmR cells. We find that the m6A level was significantly increased in BT474.TtzmR cells compared to the BT474 cells. We show that there was a close correlation between the m6A modification and herceptin resistance. Overall design: Examination of m6A modifications in 2 cell types