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Gene expression profile during odontogenic differentiation was analyzed using a microarray (miRNA).

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE138180
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The roles of microRNAs (miRNAs) in odontogenic differentiation of human dental pulp stem cells (hDPSCs) remain largely unexplored. In this study, the underlying molecular mechanism of osteogenic differentiation in hDPSCs is investigated using miRNA profifiling. Gene expression of hDPSCs at differentiated group were cultured with an odontogenic differentiation medium containing 50 mg/ml ascorbic acid, 100 nmol/L dexamethasone, and 10 mmol/L β-glycerolphosphate (Sigma, St Louis, MO, USA) for 14 days in 6-well plates. hDPSCs at undifferentiated group were cultured in 10 % FBS in DMEM with no supplements.
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2019-10-03
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