Functional Network Analysis of Fungal Pathogen Colletotrichum Sublineola Effectors in Sorghum Anthracnose - Data Tables

Table 1. Colletotrichum sublineola effectors primary data. Here we list the identified Cs effectors and describe their properties, which include amino acid sequences, architectures and initial classification according to their domains’ basic functions. These effectors are distributed in the table in three different tabs depending on their destination in the host cell as apoplastic, cytoplasmic and dual location. Table 2. A closer look at some major groups of Colletotrichum sublineola apoplastic effectors and their functional roles. This table has four Tabs: A) CFEM Tab, where we describe the CFEM proteins (effectors and non-effectors) properties inferred from their amino acid sequences, such as their domain content, probability of them being membrane proteins (according to THMM), and their predicted location in the host using the Localizer and WoLFPSORT programs. B) Glycosyl hydrolases Tab. Here we classify the GH effectors in protein families, provide their EC numbers, and predicted enzymatic activity. C) Other apoplastic enzymes Tab. We list here some CAZymes and other enzymes which are important for the digestion of the plant cell wall and other processes in the initial invasion of the host. D) Binding functions Tab. We describe here six groups of effectors with different structures and their binding ability to various substrates.Table 3. Common reactions in hydrogen peroxide metabolism. This table shows a summary of reactions listed in the BRENDA Database and is divided into two Tabs: A) H2O2 as substrate Tab, where we list enzymes that catalyze H2O2 consumption. B) H2O2 as product Tab, where we list enzymatic reactions that either the pathogen, or the host enzymes, can use to generate H2O2. Table 4. Comparative genomics of Colletotrichum sublineola proteins related to fungal pathogenicity. We used a literature search, combined with comparative genomics methodology to predict Cs proteins functions in the context of fungal pathogenicity mechanisms. We show our results in five Tabs: A) Oxidative stress response Tab, where we give examples of homologs of Cs effectors and their oxidative stress regulation functions. B) Protein modifications Tab, where we predict Cs effectors functions based on studies of their homologs in other biological systems. C) CFEM Tab, where we compare Cs CFEM protein properties with those of other organisms CFEM effectors described in the literature. D) Glycosyl hydrolases other roles Tab, where we give examples of other organisms glycosyl hydrolases with elicitor activities that do not require enzymatic activity on the cell wall. E) Protein glycosylation Tab, where we do comparative genomics of fungal enzymes related to protein glycosylation and analyze their role in fungal pathogenesis.