RNA-Seq data from selection lines of Callosobruchus maculatus

RNA-Seq data were generated from pools of adult males and females from crosses of isofemale lines of each of four selection treatments (L1, L2, L3, and SA) and one control treatment (C). Each treatment was replicated to produce two selection lines per treatment. See Kaufmann et al. (2021) for full details of selection experiments. To create the samples for RNA-Seq, the isofemale lines were first reared under density controlled conditions (by limiting oviposition window to 24h, on a surplus of beans) for two generations. Virgin adults were collected from beans that had hosted only a single egg (thus no larval competition). We then chose six isofemale lines and made three inter-line crosses. From the F1 progeny of these crosses, we picked five males and five females per cross to produce pooled samples by sex. Adult beetles were snap-frozen in liquid nitrogen 24h after eclosure. Each sequenced sample thus represents an average profile of individuals from two isofemale lines. For each selection line we produced three replicate pools for each sex, resulting in six samples representing each selection line. The total number of samples sequenced was 54 (4 selection treaments x 2 replicates x 2 sexes x 3 replicates) +(1 control treatment x 2 sexes x 3 replicates). Total RNA was extracted from whole bodies, using Qiagen RNeasy, according to manufacturer's instructions. The RNA was checked for quality and quantity using NanoDrop, Qubit, and BioAnalyzer. TruSeq stranded mRNA libraries were prepared for each sample including poly-A selection to enrich for mRNA. Paired-end sequencing for read lengths of 150bp was performed on a single NovaSeq 6000 S4 flowcell. Library preparation and sequencing was performed by the SNP & SEQ Technology Platform of SciLifeLab in Uppsala.ReferencesKaufmann et al. 2021. Nature Ecology and Evolution. 5: 1394-1402. https://doi.org/10.1038/s41559-021-01530-z.