Parallel reaction monitoring using quadrupole-orbitrap mass spectrometer: principle and applications

The PRM dataset corresponds to the raw data files supporting the comparison between conventional database search using Mascot and spectral matching strategy (Figure 3). The PRM and DDA raw data files support the comparison between these two acquisition methods using the spectral matching strategy to corroborate the identity of the peptides (Figure 4D). The data were generated from the triplicate analyses of a dilution series of 778 SIL peptides spiked in a yeast tryptic digest at concentrations ranging from 50 amol/µL to 37 fmol/µL.