Brown adipose tissue and skeletal muscle coordinately contribute to thermogenesis in mice

Endotherms increase the rate of metabolism in metabolic organs as one strategy to cope with a decline in the temperature of the external environment. However, an additional major contributor to maintenance of body temperature in a cold environment is contraction-based thermogenesis in skeletal muscle. Here, we show that impairment of hind limb muscle contraction by cast immobilization induced a loss of function of skeletal muscle and activated brown adipose tissue (BAT) thermogenesis as a compensatory mechanism. BAT utilizes free branched-chain amino acids (BCAAs) derived from skeletal muscle as an energy substrate for thermogenesis, and interleukin-6 released by skeletal muscle stimulates BCAA production in muscle for support of BAT thermogenesis. Additionally, this thermoregulatory system between BAT and skeletal muscle may also play an important role in response to cold temperatures or acute stress. Our findings suggest that BAT and skeletal muscle cooperate to maintain body temperat..., , , # Data from: Brown adipose tissue and skeletal muscle coordinately contribute to thermogenesis in mice

Dataset DOI: [10.5061/dryad.18931zd9c](https://doi.org/10.5061/dryad.18931zd9c)

## Description of the data and file structure

This dataset was generated to investigate physiological compensatory mechanisms associated with skeletal muscle dysfunction induced by hindlimb immobilization in male C57BL/6J mice. We analyzed metabolic changes in skeletal muscle and brown adipose tissue (BAT) using CE-TOFMS–based metabolomics, real-time PCR for gene expression, and radiolabeled tracer experiments to examine amino acid dynamics. To explore the role of interleukin-6 (IL-6) as a regulatory factor in muscle–BAT crosstalk, similar analyses were performed in IL-6 knockout mice.

### Files and variables

**Figure_1_source_data_1.xlsx**

**Description:** 

**Figure 1:** 

(A) Time course of rectal core body temperature of cast-immobilized (gypsum, n = 6) and control (n = 5) mice subjected to a cold...