Infant gut microbiome sequencing study including mock controls, technical replicates and stool/rectal swab sample pairs

This dataset contains mock controls, replicates and pairs of stool and swab samples from the same participants. Sequencing was done using xGen 16S rRNA v2 Amplicon Panel kits and an Illumina Miseq platform, which enable short-read sequencing of all nine variable regions of the 16S rRNA gene. The goal was to process this data using the corresponding SNAPP-py3 pipeline, to assess how well the sequencing kits and pipeline could perform species-level classification. Futhermore, we sought to determine whether this methodology could achieve the same results for stool and rectal swab samples collected from the same participants. The aim of this quality control assessment is to set the stage for future analysis of infant gut microbiome samples to a species level using this short-read multiple variable region sequencing approach.