Identification of MELK-regulated genes in prostate cancer cells using next-generation sequencing

A cross-species analysis identified MELK as a potential therapeutic target in prostate cancer. To further elucidate the functional role of MELK in prostate cancer cells, we aimed to identify MELK-regulated genes. C4-2b cells were either treated with a small-molecule MELK inhibitor (OTSSP167), or transfected with siRNAs targeting MELK. Differentially expressed genes were identified using next-generation sequencing. Our results demonstrate that MELK promotes the expression of genes associated with tumour progression in prostate cancer cells. Gene expression profiles of C4-2b cells by RNA-Seq following silencing or inhibition of MELK. Four independent biological replicates were performed for each treatment condition. Two different siRNAs directed against MELK were used to account for potential off-target effects of the siRNAs. Negative controls were cells transfected with AllStars Negative Control siRNA, and cells treated with DMSO (vehicle for OTSSP167).