Gene expression profiling of total rat epididymal cells during postnatal development.

Immature epididymis is formed of undifferentiated columnar cells which differentiate into the different cell types forming the mature epithelium. Our goal was to assess the gene expression profiles at different time points in the developping epididymis. RNA was isolated from pools of whole epididymides of 7- (n=3), 18- (n=4), 28- (n=4) and 60-day (n=3) old rats and hybridized on Affymetrix RatGene 2.0 ST array.