The levels of each immune subset in splenocytes and TILs (MC38).

Spleens were filtered through mesh, and live splenocytes were collected by 100% Ficoll gradient centrifugation. Tumor tissues were dissected and digested, and filtered through mesh, and TILs were collected by 80%/100% Ficoll gradient centrifugation. Splenocytes or TILs were stained with anti-CD4, anti-IFN-γ, anti-IL-4, anti-Foxp3, anti-Gr-1, anti-CD11b and anti-CD45 mAb. Data are representative of two independent experiments. Values represent the mean percentage ± SE in CD4+ or CD45+ population. (n = 5 per group, *Pt test), compared with Ad-SNC).