Dataset for the article "Nitrogen-doped carbon quantum dot enhance bacteria growth and biosensing signals"

Description for the dataset of article 'Nitrogen-doped carbon quantum dot enhance bacteria growth and biosensing signals'

David Rutherford*1, Marketa Šlapal Bařinková1, Jaroslav Kuliček1, Jelena Kozic2, Jovana Prekodravac Filipović2, Bohuslav Rezek1

1 Faculty of Electrical Engineering, Czech Technical University in Prague, Prague, Czech Republic 

2 Vinča Institute of Nuclear Sciences-National Institute of the Republic of Serbia, University of Belgrade, Serbia.

Journal: Nanoscale

'CQD_RAW' contains the raw data for all bacteria growth measurements, photoluminescence spectra acquisition and UV-vis spectrocopy analysis. - 'MIC' tabs = bacteria growth- 'PL' tabes = photoluminescence spectroscopy- 'UV' tabs = UV-vis spectroscopy

Figures- Figure_CQD_bacteria growth.tif = composite images of bacteria growth parameters as a function of CQD type and concentration.a) lag phase length, b) maximum growth rate, c) time to maximum growth rate, d) final cell concentration- Figures_CQD_PL_bact.tif = a) photoluminescence spectra of CQD samples with and without bacteria b) percentage of N-CQD PL signal quenching as a funstion of difference bacteria concentrations.- Figures_CQD_uv.vis_stab.tif = a) optical absorbance spectra of CQD samples in water and NaCl. b) measurement of the absorbance value at 230 nm over time  

Carbon quantum dots (CQDs) are known for their antibacterial properties and ability to inhibit bacteria growth. In the current study, we observed a dopant and concentration-dependency on the antibacterial effect of CQDs. High concentrations of CQDs completely inhibited bacteria growth yet low concentrations enhanced growth. Unlike undoped CQDs, nitrogen-doped CQDs (N-CQDs) enhanced bacteria growth in a concentration-dependant manner and nitrogen/iron co-doped CQDs (Fe/N-CQDs) resulted in growth profiles similar to untreated bacteria. N-CQDs also exhibited the strongest photoluminescence (PL) signal which was quenched by bacteria, and the reduction in the maximum PL intensity was linear over the concentration range tested until saturation. N-CQDs were found to be located around the bacteria cell periphery suggesting intimate interactions. Illumination prior to interaction with bacteria had little influence on these growth effects. Absorbance measurements of colloidal CQD, N-CQD and Fe/N-CQD confirmed long-term stability (7 days). Such materials have potential for incorporation into rapid sensing and diagnostic systems for bacteria detection in liquids for biomedical applications.