Bacteria regulate B cell function in the gut

B cell plays an important role in antibody induction response. We were aiming to describe two distinct B cell subpopulations in the intestinal lymphoid tissue ‘Peyer’s patches’ of mice, characterized by their transcriptional profile. B cells were sorted according to a combination of surface marker from wild type young mice, that were treated with bacteria for 7 days or control. RNA was extracted. Microarray analysis was then applied to identify the difference between two B cell subsets and the changes of expression within each B subset, due to treatment. To better understand the signaling events that separate BB and SB cells in PPs, four replicates of BB and SB from WT control mice or bacteria–treated mice were analyzed by whole-mouse genome microarray.