Morphology and ultrastructure of external sense organs of Drosophila larvae

Sensory perception is the ability through which an organism is able to process sensory stimuli from the environment. This stimulus is transmitted from the peripheral sensory organs to the central nervous system, where it is interpreted. Drosophila melanogaster larvae possess peripheral sense organs on their head, thoracic, and abdominal segments. These are specialized to receive diverse environmental information, such as olfactory, gustatory, temperature, or mechanosensory signals. In this work, we complete the description of the morphology of external larval sensilla and provide a comprehensive map of the ultrastructure of the different types of sensilla that comprise them. This was achieved by 3D electron microscopic analysis of partial and whole body volumes, which contain high-resolution and complete three-dimensional data of the anatomy of the sensilla and adjacent ganglia. Our analysis revealed three main types of sensilla on thoracic and abdominal segments: the papilla sensillum,..., Data collection was focused on Ion-Beam electron microscopy with minimal embedding technique (see manuscript material and methods)., , # Morphology and ultrastructure of external sense organs of Drosophila larvae

[https://doi.org/10.5061/dryad.1vhhmgr1z](https://doi.org/10.5061/dryad.1vhhmgr1z)

This is a collection of focus ion-beam SEM datasets of the external sense organs of Drosophila larvae. The specimens used were third-instar wildtype Canton-S larvae.

## Description of the data and file structure

In the Zip folder 'EM_data_zipped', there are two main folders, one with the focused Ion-Beam scanning electron microscopy (**FIB-SEM**) data and the other one with the scanning electron microscopy (**SEM**) data of the outer morphology of the different sensilla. Each main folder contains subfolders for the sensilla investigated in this work.

For each sense organ or sensillum type, there are one or more FIB-SEM volumes. Furthermore, for each sensillum, there is at least one SEM image for the outer morphology.

For metadata, open file in Fiji or text editor --> FOV_X units is the width of the image in um. FOV_Y un...