Expression data from mouse aorta with chronic intermittent hypoxia exposure. Expression data from mouse aorta with chronic intermittent hypoxia exposure

Atherosclerosis is the pathological basis of cardiovascular disease. Obstructive sleep apnea aggravates atherosclerosis, and chronic intermittent hypoxia (CIH) as a prominent feature of obstructive sleep apnea plays an important role during the process of atherosclerosis. The mechanisms of CIH in the development of atherosclerosis remain unclear. The microarray was used to investigate differentially expressed mRNAs and long noncoding RNAs (lncRNAs) in aorta from five groups of ApoE-/- mice fed with a high-fat diet and exposed to various conditions: normoxia for 8 weeks, CIH for 8 weeks, normoxia for 12 weeks, CIH for 12 weeks, or CIH for 8 weeks followed by normoxia for 4 weeks. Overall design: For microarray profiling, the mouse aorta total RNA was harvested using TRIzol reagent (Life Technologies, CA, USA) and purified using RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. The amount and quality of RNA were determined using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, MA, USA). The mRNA expression profiling was measured using Affymetrix ClariomTM D mouse Array (Affymetrix, Thermo Fisher Scientific), which contains 65956 gene-level probe sets.

动脉粥样硬化是心血管疾病的病理基础。阻塞性睡眠呼吸暂停可加重动脉粥样硬化进程，而作为阻塞性睡眠呼吸暂停典型特征的慢性间歇性缺氧（chronic intermittent hypoxia, CIH）在动脉粥样硬化发生发展中发挥重要作用，但其具体调控机制尚未明确。
本研究采用微阵列技术，对5组经高脂饮食喂养、接受不同处理的ApoE-/-小鼠（ApoE-/- mice）的主动脉组织进行差异表达mRNA及长链非编码RNA（long noncoding RNAs, lncRNAs）分析。5组处理条件分别为：常氧暴露8周、慢性间歇性缺氧暴露8周、常氧暴露12周、慢性间歇性缺氧暴露12周，以及先经慢性间歇性缺氧暴露8周后再常氧恢复4周。
实验整体设计如下：进行微阵列检测前，采用TRIzol试剂（TRIzol reagent，Life Technologies，美国加利福尼亚州）提取小鼠主动脉总RNA，并依照制造商说明书使用RNeasy Mini Kit（RNeasy Mini Kit，Qiagen，德国希尔德）完成RNA纯化。使用NanoDrop 1000分光光度计（NanoDrop 1000 spectrophotometer，Thermo Fisher Scientific，美国马萨诸塞州）检测RNA的浓度与质量。mRNA表达谱分析采用Affymetrix Clariom™ D小鼠基因芯片（Affymetrix Clariom™ D mouse Array，Affymetrix，赛默飞世尔科技），该芯片共包含65956个基因水平探针组。

搜集汇总

背景与挑战

背景概述

该数据集提供了小鼠主动脉在慢性间歇性缺氧暴露下的表达数据，旨在研究慢性间歇性缺氧在动脉粥样硬化发展中的作用。通过使用ApoE-/-小鼠模型和微阵列技术，分析了差异表达的mRNA和长非编码RNA，涉及多个时间点的实验组别，以探索缺氧对心血管疾病的潜在机制。