Multi-omics analysis revealed the potential role of rumen microbes in heat stress

Heat stress has been an important issue in dairy cattle feeding management affecting summer health and economic efficiency. In recent years, global climate change has led to an increase in atmospheric CO2 and average daily temperature, making heat stress a major challenge in dairy farming. In this experiment, 10 cows each of growing heifers, heifers, and lactating cows were selected for sample collection in April and August , respectively. Rumen fluid were collected, and gauze filtered to obtain rumen fluid immediately transferred to a liquid nitrogen tank for measurement of 16SrDNA. Here, we characterized both the changes in rumen microbial communities potential derived from rumen fluid samples in cows during different growth stages and climates.