Controlled irrigation suppresses methane emissions by reshaping the rhizosphere microbiomes in rice

The rhizosphere microbiomes of rice plants under conventional flood irrigation consist of highly complex consortia of microorganisms, and in particular, methanogens purportedly associated with methane emissions therein. Controlled irrigation has been proposed as a cultivation method of choice over continuous flooding to reduce water and fertilizer usage in an aerobic environment. However, a systemic understanding of the assembly and function of microbiota in the rhizosphere under drip and flood irrigation remains unclear. Using empirical analyses, we report a significant reduction in methane emissions in controlled irrigation compared to the flooded environment. Genotypic or varietal differences did not influence such methane emissions under conventional flooded cultivation of rice. Using metagenomic sequencing and computational analyses, we provide a deeper understanding of how drip irrigation or continuous flooding affects the root-associated microbiomes in rice. Rhizosphere soil from..., , # Data from: Controlled irrigation suppresses methane emissions by reshaping the rhizosphere microbiomes in rice

Dataset DOI: [10.5061/dryad.76hdr7t8s](https://doi.org/10.5061/dryad.76hdr7t8s)

## Study Description

In this study, we assessed and characterized the soil microbiomes of two indica rice varieties, Huanghuazhan (HHZ, China) and Temasek rice (Singapore) under drip irrigation and continuous flooding conditions in a greenhouse. The microbiome composition and structure helped provide further insight on the possible microbial activities and metabolic functions in the soil. In addition, potential interactions among different microbial species/taxa were inferred using microbial co-occurrence network analyses. Important keystone members were identified to predict microbial clusters and their relation to methane emissions. HHZ is an elite indica rice variety from South China that is known for its high yield. Temasek rice is a variety bred using marker-assisted breeding technique per...,