Gene expression changes in rat islets with glucolipotoxic conditions with or without protective compound. Gene expression changes in rat islets with glucolipotoxic conditions with or without protective compound

To determine which GLT-regulated genes might be affected by compound D, we performed an RNA-sequencing analysis of rat islets treated ex-vivo with or without GLT and compound D for 3 hours and 24 hours. Compound E (related inactive compound) was used to account for non-specific scaffold effects. Overall design: Islets from 8 rats were pooled together and divided into 8 groups: 3hr GLT (27mM glucose, 300uM PA); 3hr control (11mM glucose, no PA); 24hr GLT;24hr control; 3hr GLT + cpd D 300nM; 24hr GLT + cpd D 300nM; 3hr GLT + cpd E 300nM; 24hr GLT + cpd E 300nM. After treatment islets were collected for RNA prep; Experiments were repeated 3 times (3 separate isolations)