Effect of dietary supplementation with Chlorella vulgaris and lysozyme on the microflora of the colon of weaned piglets

The experiment was carried out from December 2023 to February 2024 at the Institute of Subtropical Agricultural Ecology of the Chinese Academy of Sciences, and 30 Xiangcun black pigs weighing 11.52 ± 1.13 kg, half males and half females, were randomly divided into the control group, the Chlorella group, and the Chlorella + lysozyme group, and were fed with the basal diet, the basal diet + 5% Chlorella, the basal diet + 5% Chlorella + 100 mg /kg lysozyme, with 10 replicates in each group and 1 pig in each replicate. The pre-test period was 3 d and the formal period was 56 d. One pig died in each of the three groups, so there were 9 samples in each group, in which CRTL represented the control group, CHV represented the Chlorella group, and LYS represented the Chlorella+lysozyme group, and 540 samples of each of the top 20 phyla and phyla were selected at both phylum and genus levels, and 27 samples of each of the α and β diversity, and 1336 samples of each of the top 20 phyla and phyla were also selected for LEfSe analysis. 27 samples totaling 1336 data.