poly(A+) RNAs 3'-seq analysis in the cytoplasmic RNAs of control and U1 AMO treated Hela cells

To understand if U1-AMO induced truncated forms of mRNAs could be exported into cytoplasm, we performed 3'-seq analysis in the cytoplasmic RNAs of control and U1 AMO treated Hela cells Control and U1 AMO were transfected into Hela cells, cytoplasmic fractions were isolated and RNAs were subsequently purified by Trizol reagent. 3'-seq analysis was performed using lexogen kit (016.024)