CD3+ cells produce cytokines and chemokines after 4 hours of co-culture with H. pylori.

Purified CD3+ cells from peripheral blood of H. pylori negative donors produce a wide range of cytokines and chemokines after 4 h of culture with H. pylori. Culture supernatants was collected and analyzed by bioplex assay. Data represent the mean and the range of cytokines and chemokines produced by T cells. Statistical significances of the differences between the cytokines production of CD3+/H. pylori co-culture compared to the control group (medium) were assessed using paired t test and reported in the third column. The differences in cytokines production in presence of H. pylori wt and ΔCagA were also calculated and compared, as reported in the last column of Table 1. The average was calculated from four independent experiments. Statistical significance was determined by Student's paired T-test at *: P≤0.05. Note: n.d = not detectable. n.a = not applicable.