TGFβ/Cyclin D1/Smad-mediated inhibition of BMP4 promotes breast cancer stem cell self-renewal activity

To understand the mechanism by which TGFβ regulates tumor initiation in triple negative breast cancer (TNBC), we performed microarray analysis using Illumina Human HT-12 Gene Expression BeadChip. A TNBC cell line of SCP2 was treated or not with TGFβ for 24 hours. Differentially expressed genes were analyzed by comparing treated versus non-treated samples Triple negative breast cancer cells, SCP2, were serum-starved for overnight and treated or not with 100 pM TGFβ for 24 hours in serum-free medium. Total RNA samples were extracted using the TRIzol reagent (Invitrogen).