RNA-seq in Prdm16 KO doudenal and villi

RNA was extracted from duodenal villi from crontrol and Prdm16 KO mice 3 days after deletion RNA was isolated from duodenal villi of WT (Prdm16loxP/loxP) or KO (Rosa26CreERT2; Prdm16loxP/loxP ) mice 3 days after tamoxifen injection. Total RNA was extracted from isolated crypts or villi using TRIzol (Invitrogen) combined with Purelink RNA Mini columns (Fisher). RNA-seq libraries were generated using a Truseq RNA Sample Preparation kit (Version 2, Illumina, RS-122-2001). RNA-seq was performed by the University of Pennsylvania Next Gen Sequencing Core using a HiSeq 2000 high output sequencer. Differential expression was determined using the EdgeR software package using log2 fold change of KO divided by WT. 0-1 False Discovery Rates were calculated from the p-value using a Benjamini-Hochberg correction.