Apparent kinetic parameters of KMTB production using wild type and mutant l-AAD containing whole-cell biocatalysts.
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aThe volume of each reaction mixture was 20 ml, and amount of biocatalyst in the reaction solutions was equal in quantity. All experiments were carried out at 40°C in 20 mL of 50 mM Tris–HCl (pH 8.0). Km values are expressed in terms of mM of substrate.Apparent kinetic parameters of KMTB production using wild type and mutant l-AAD containing whole-cell biocatalysts.
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2015-12-02
