RNAseq analysis of skeletal muscle transcriptomes from wild type and MLL4SET-MKO mice

This experiment was conducted to identify mRNA transcripts alteration in skeletal muscle of MLL4SET muscle-specific knockout mice. Enhancers play central role in controlling gene expression in time and space. Primed enhancers are marked by histone H3 lysine 4 (H3K4) mono/di-methylation (H3K4me1/2). Mixed-lineage leukemia 4 (MLL4/KMT2D) is an evolutionarily conserved H3K4me1/2 methyltransferase that is required for enhancer activation. Here, we identified the genome-wide MLL4 occupancy in mouse skeletal muscle by ChIP-seq coupled with RNA-seq analysis. Gene ontology analysis revealed that MLL4 controls muscle fiber-type switching. We thus have revealed novel MLL4 targets involved in muscle metabolism. Overall design: Gastrocnemius muscle mRNA profiles of 8-week-old WT and MLL4SET-MKO mice were generated by deep sequencing, in triplicate, using Illumina HiSeq 4000.