The master transcription factor of the Plasmodium merozoite formation. [ChIP-Seq]

To investigate the genome-wide binding sites of PbSIP2 in Plasmodium berghei, the chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) analyses were performed. Parasites expressing GFP-fused PbSIP2 (PbSIP2::GFP) in cultures were harvested at 24 h post infection and subjected to ChIP-seq experiments using anti-GFP antibody. Binding sites of PbSIP2 were determined from the sequence data. Overall design: PbSIP2::GFP parasites were synchronized by injecting cultured schizonts, which were harvested by density gradient centrifugation, into mice. Chromatins associated with GFP-fused PbSIP2 were IPed with anti-GFP antibody, and DNA fragments purified from the IPed chromoatins were sequenced by the Next Generation Sequencing. Using non-IPed samples as a control (INPUT), binding sites of PbSIP2 were determined from the sequence data.