Proteomic insights into the effects of cryopreservation on sperm of endangered Eld’s deer

The success of fertilization largely depends on semen quality. However, semen cryopreservation may lead to reduced sperm function and decreased conception rates, posing a difficulty to the re population of threatened species, including Eld's deer (Rucervus eldii). Despite its importance, knowledge of the effects of cryopreservation on sperm protein composition in Eld’s deer remains limited.