Effect of enzymes blend supplementation in a diet containing distillers dried grains with soluble for broiler chickens

A total of 288 one-day-old by-product day-of-hatch broilers chickens were assigned to six treatments. The treatments differed by the inclusion of DDGS in the diet fed to the birds (0, 7 and 14% inclusion of DDGS) and by the inclusion of exogenous enzymes (with or without a multi-carbohydrase complex + phytase (MCPC)) using a completely randomized 3x2 (3 DDGS inclusion X 2 enzyme inclusion) factorial design. Necropsy was performed on day 28, when one chicken from each pen (8 per treatment) were randomly selected and euthanized by cervical dislocation. During the necropsy, samples of ileal and cecal content were collected for microbiota assessment.