Effect of FGF10 on human Embryonic Stem Cells (hESC) and induced Pluripotent Stem Cells (hiPSC)

We wish to assess the transcritpomic regulation of FGF10 on human Embryonic Stem Cells (hESC) and human induced Pluripotent Stem Cells (hiPSC) that have been induced to express the earliest lung progenitor genetic marker, NKX2.1. Shortly after NKX2.1 was expressed in these cells, they were reseeded and FGF10 was added to the culture medium (experimental conditions) for either 12 or 24 hrs. Control samples had no FGF10 added. After the desired time, cells were harvested and stored in Trizol lysis buffer. RNA was extracted using a miRNeasy mini kit, and used for gene array analysis. Since this is a pilot experiment, we only have n=1 for experimental samples and n=1 for control samples for each condition. 8 groups, each with 1 biological replicates