Nuclear noncoding RNAs are key regulators of gene expression and chromatin organization. Here we present RedChIP, a technique combining RNA-DNA proximity ligation and chromatin immunoprecipitation for
F indicates the forward primer and R the reverse. Source shows the literature source the primer was taken from or the NCBI genome reference used to design primers. (XLSX)
A specialized ChIP protocol to facilitate the investigation of protein-DNA interactions in E. diaphana gene regulation is presented. The cross-linkingand chromatin extraction steps were optimized for