Room-temperature X-ray structure of E53Q mutant of Thermus thermophilus serine hydroxymethyltransferase (TthSHMT) in complex with PLP-L-Ser external aldimine and tetrahydrofolate (THF)

Room-temperature X-ray structure of E53Q mutant of Thermus thermophilus serine hydroxymethyltransferase (TthSHMT) in complex with PLP-L-Ser external aldimine and tetrahydrofolate (THF) Descriptor: (6S)-5,6,7,8-TETRAHYDROFOLATE, (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine, ACETATE ION, ... Authors: Kovalevsky, A, Drago, V.N, Phillips, R.S. Deposit date: 2026-02-12 Release date: 2026-05-20 Last modified: 2026-05-27 Method: X-RAY DIFFRACTION (1.8 Å) Cite: Mutation of active site glutamate in serine hydroxymethyltransferase allows trapping a reactive intermediate: a combined neutron and X-ray crystallography study. Rsc Adv, 16, 2026