Optimisation of HTT-HAP40 purification using heparin affinity chromatography - 2019/01/29

<strong>Project: </strong>Biophysical investigation of purified HTT protein samples <strong>Experiment: </strong>Optimisation of HTT-HAP40 purification using heparin affinity chromatography <strong>Date completed:­ </strong>2019/01/29 <strong>Rationale: </strong>The current protocol for HTT and HTT-HAP40 purification I am using requires a long incubation of clarified cell lysate with FLAG resin. To potentially improve yields and sample quality, it would perhaps be beneficial to have a quick heparin resin purification step prior to FLAG binding which may also remove contaminating nucleic acid material. To test this hypothesis, small-scale purification of Q23 HTT-HAP40 samples in different buffer systems were conducted using heparin and FLAG affinity chromatography which showed the sample bound heparin resin – see https://zenodo.org/record/2553669. Now this need to be scaled up and tested more stringently.