Data_Sheet_1_Application of High-Throughput Sequencing on the Chinese Herbal Medicine for the Data-Mining of the Bioactive Compounds.PDF

The Chinese Herbal Medicine (CHM) has been used worldwide in clinic to treat the vast majority of human diseases, and the healing effect is remarkable. However, the functional components and the corresponding pharmacological mechanism of the herbs are unclear. As one of the main means, the high-throughput sequencing (HTS) technologies have been employed to discover and parse the active ingredients of CHM. Moreover, a tremendous amount of effort is made to uncover the pharmacodynamic genes associated with the synthesis of active substances. Here, based on the genome-assembly and the downstream bioinformatics analysis, we present a comprehensive summary of the application of HTS on CHM for the synthesis pathways of active ingredients from two aspects: active ingredient properties and disease classification, which are important for pharmacological, herb molecular breeding, and synthetic biology studies.

中医中药（CHM）在全球范围内广泛应用于临床治疗人类绝大多数疾病，其疗效显著。然而，中药的功能成分及其相应的药理机制尚不明确。作为主要手段之一，高通量测序（HTS）技术已被广泛应用于发现和解析CHM中的活性成分。此外，大量努力被投入以揭示与活性物质合成相关的药效基因。在此，基于基因组组装和下游生物信息学分析，我们全面概述了HTS在CHM中的应用，从活性成分特性与疾病分类两个方面探讨了活性成分合成途径，这对于药理学、草本分子育种和合成生物学研究具有重要意义。