Test for acclimation or adaptation of T. pseudonana after 3 months continuous culture in media at 760 µatm pCO2 or present-day conditions of 380 µatm pCO2.

760→760 - cells from the long-term culture grown at 760 µatm were used to inoculate a batch culture equilibrated with 760 µatm CO2 aq media: 760→380 - the same cells (760) inoculated culture media equilibrated with air (380): 380→760 - cells from long-term culture equilibrated with air were inoculum for a batch culture equilibrated with 760 µatm CO2 aq media: 380→380 – air grown cells inoculate air-equilibrated batch culture. All data are means (±SD) evaluation experiment. Flow cytometry readings for red fluorescence (Red fl), side scatter (SSC) and forward scatter (FSC) per cell, determinations of Fv/Fm, σ, C:N ratio.