Long noncoding RNA lnc-H2AFV-1 promotes cell growth by regulating aberrant N6-methyladenosine RNA modification in head and neck squamous cell carcinoma

Head and neck squamous cell carcinoma (HNSCC) is the most common malignant tumor in the oral and maxillofacial regions, and long noncoding RNAs (lncRNAs) play crucial roles in the occurrence and progression of HNSCC. in our study, quantified m6A RNA methylation and dot blot assays revealed that total m6A methylation in HNSCC cells was accompanied by lnc-H2AFV-1 expression. Western blotting showed that the expression of methyltransferase-like 3 (METTL3) and 14 (METTL14) was consistent with that of lnc-H2AFV-1, whereas the expression of demethylase fat mass and obesity-associated (FTO) was contrary to that of lnc-H2AFV-1. Methylated RNA immunoprecipitation sequencing (MeRIP-seq) and MeRIP-qPCR were used to determined the target genes regulated by lnc-H2AFV-1 overexpression in HNSCC cells. Examination of m6A modificated genes in HN6-LV-H2AFV-1 cells and HN6-LV-NC cells.