Apoptosis-related gene expression profiling in hematopoietic cell fractions of MDS patients. Homo sapiens

Quantification of apoptosis-related genes in bone marrow HSPCs from MDS patients compared to healthy donor by microfluidic cards Overall design: Bone marrow from MDS patients and healthy controls was collected. The expression of 380 different apoptosis-related genes was measured simultaneously by real-time PCR employing a 384 Microfluidic Card (Applied Biosystems). cDNA obtained from the CD34+, CD71+ and CD13/33+ cell fractions was analyzed. The relative expression of the apoptosis-related genes was calculated by measuring the housekeeping gene GAPDH.