Integrative Microbiome and Metabolome Analyses Reveal the Effect of Microbial Fertilizer on Microbial Community Diversity and Metabolic Cycle in Rhizospheric Soil Under Tobacco Monoculture

Over application of chemical fertilizers and continuous cropping obstacles seriously restrict the sustainable development of tobacco production. Localized fertilization of beneficial microbes has potential advantages in achieving higher productivity and nutrient use efficiency, but the underlying biological mechanisms of interactions between rhizospheric microorganisms and the related metabolic cycle remain poorly characterized. Here an integrative analysis of microbiomes with non-targeted metabolomics was performed on 30 soil samples of rhizosphere, root surrounding, and bulk soils from flue cured tobacco under continuous and noncontinuous monocropping systems. The analysis was conducted using UPLC MS platforms and high-throughput sequencing targeting the bacterial 16S rRNA gene and fungal ITS gene.