大米草和互花米草快速鉴别数据

由于互花米草和大米草基因组庞大，分别为六倍体和十二倍体，并且互花米草是大米草的母系来源，因此使用叶绿体基因片段无法区分两个物种，而微卫星等传统分子标记在多倍体材料中又普遍存在实验结果一致性较差，假阳性率高，信息损失等问题。并且因杂交起源，大米草ITS及部分核基因序列的一代测序结果更是存在套峰严重，无法准确判定位点碱基等负面因素。本技术针对现有技术中存在的上述问题，利用高通量简化基因组测序技术（RAD-seq），筛选出互花米草和大米草具有稳定差异的DNA片段，并以此为基础所开发的分子标记，能够准确、简单、快速的鉴定互花米草和大米草。

Due to the large genome sizes of Spartina alterniflora and Spartina anglica, which are hexaploid and dodecaploid respectively, and the fact that S. alterniflora is the maternal progenitor of S. anglica, chloroplast gene fragments cannot be used to distinguish these two species. Furthermore, traditional molecular markers such as microsatellites commonly suffer from poor experimental reproducibility, high false positive rates and information loss when applied to polyploid materials. Additionally, due to their hybrid origin, Sanger sequencing results of the ITS region and some nuclear gene sequences of S. anglica present severe overlapping peaks, making it impossible to accurately determine the nucleotide bases at each locus. To address these aforementioned issues in existing technologies, this method utilizes high-throughput reduced-representation genome sequencing (RAD-seq) to screen for DNA fragments with stable differences between S. alterniflora and S. anglica. The molecular markers developed based on these fragments can accurately, simply and rapidly identify the two Spartina species.

搜集汇总

背景与挑战

背景概述

该数据集专注于大米草和互花米草的快速分子鉴别，针对这两种植物因基因组复杂性和杂交起源导致的传统鉴定方法局限性，采用高通量简化基因组测序技术（RAD-seq）开发稳定差异的DNA标记，以实现准确、高效的物种区分。数据集属于滨海滩涂湿地生态恢复项目，旨在支持生态恢复技术研究，数据量较小（13.9 MB），适用于植物学领域的科研应用。