Data from: Low bottleneck detection in long-lived species despite lost genetic diversity: a case study of tuatara and eastern massasauga rattlesnakes

The original/raw tuatarta genotypes were obtained at seven microsatellite loci collected from 272 individuals sampled from Takapourewa/Stephens Island, New Zealand between March 2003–March 2007. Sampling occurred at eight sites on the island and was distributed throughout the available remnant forest and pasture landcover types. For simulated tuatara and eastern massasauga microsatellite genotypes we used program BOTTLESIM to to simulate multiple bottleneck scenarios for each species. For all simulations, we performed 1,000 iterations and set the reproductive system as dioecy with random mating, a 1:1 sex ratio, and 100% generational overlap. For simulation dataset 1, simulations varied by 1) length of time (tB): 50, 100, and 200 years, 2) starting population size (N0): estimated census population size and one order of magnitude above and below, and 3) bottlenecked population size (NB): 20, 50, 100, 1000, and N0 (the lattermost NB = N0 scenario representing a constant population size with no bottleneck). For eastern massasauga simulations, we assumed a census population size of 6,300 individuals, age of sexual maturity of 5 years, and longevity of 12 years. For tuatara simulations, we assumed a census population size of 25,000 individuals, age of sexual maturity of 14 years, and longevity of 80 years. For simulation dataset 2, we randomly selected seven loci from the eastern massasauga dataset. Using this reduced dataset, we conducted a set of simulations with the same values for tB and NB as described above and set N0 equal to estimated census population size. For simulation dataset 3, we simulated bottlenecks using the values for tB and NB described above and set N0 equal to estimated census population size for each species. Age of sexual maturity and longevity were set at 10 years and 50 years, respectively, for both species.