Transcriptomic analysis of C. elegans mutants which fail to activate the oomycete recognition response. Transcriptomic analysis of C. elegans mutants which fail to activate the oomycete recognition response

We used RNA-seq to identify gene expression changes in C. elegans old-1(ok1273) and flor-1(icb93) mutants along with old-1 overexpression transgenic strains Overall design: Comparative gene expression of oomycete extract treated L4 staged N2, old-1(ok1273) and flor-1(icb93) animals with untreated controls, along with animals overexpressing old-1 under its endogenous promoter (+old-1p::old-1) compared with non-transgenic animals from the same population as control; and animals overexpressing old-1 under epidermal promoter (+dpy-7p::old-1) compared with animals carrying the co-injection marker (hygromycin resistance) as control.