Summary of DNA constructs generated.

Plasmids generated here to express transgenes in T. brucei. Indicated is the promoter upstream of the transgene cassette (tetracycline-inducible promoters are indicated with Tet Op); the integration target (the genomic sequence into which the construct should be targeted by homologous recombination); the resistance marker genes used to select for transformants (conferring either hygromycin or puromycin resistance); the parent construct and the inserted sequence. Further details of the construction and sequences of these plasmids are available upon request.